The Prognostic Performance of Lung Diffusing Capacity in Preserved Ratio Impaired Spirometry: An Observational Cohort Study

Introduction

Preserved ratio impaired spirometry (PRISm), also referred to as restrictive pattern or unclassified spirometry, is a clinical entity associated with an increased risk of developing chronic obstructive pulmonary disease (COPD).^1,2 Epidemiological studies worldwide revealed that 3.7–22.3% of the general population were compatible with PRISm,^3–11 indicating that PRISm is a common disease condition. Considering its high prevalence and clinical aspects as a prodromal phase of COPD, PRISm, whose pathophysiology is still largely unknown, is an increasing threat to public health. Since the diffusing capacity of the lung for carbon monoxide (D_LCO), a useful biomarker for evaluating the gas transfer properties of the respiratory system,¹² is generally deficient in COPD,¹³ it might be also impaired in cases of PRISm. However, this issue has not yet been investigated.

PRISm has been demonstrated to be associated with an increased risk of premature mortality,^2,3,11,14 although little is known about the predictive biomarkers for death in PRISm. Since a deficit in D_LCO is a strong risk factor for a poor prognosis in COPD,¹⁵ D_LCO may also be inversely associated with morbidity and mortality in PRISm. However, there has been no study assessing the influence of D_LCO on mortality in subjects with PRISm; therefore, the verification of this hypothesis could be of great benefit for improving the clinical management for such patients.

Based on these considerations, we conducted the present study to evaluate the prevalence of reduced D_LCO in subjects with PRISm and to compare it with that in cases of normal spirometry or COPD. We also assessed the clinical implication of D_LCO impairment as a biomarker for all-cause mortality among subjects with PRISm.

Materials and Methods

Study Population

The current study was conducted as an observational cohort study through a review of medical records at the National Hospital Organization Fukuoka National Hospital. The entire cohort consisted of 899 patients ≥ 40 years of age who had a D_LCO assessment from June 1, 2017, to May 31, 2020, regardless of department, with complete information on all relevant covariates. We classified the subjects into five groups based on the results of spirometry: preserved spirometry, PRISm, mild COPD, moderate COPD, and severe/very severe COPD. PRISm was defined as the coexistence of two major criteria: (i) post-bronchodilator forced expiratory volume in 1 second to forced vital capacity (post-BD FEV₁/FVC) ≥ 70%; and (ii) reduction in FEV₁, that is, post-BD FEV₁ per predicted (FEV₁% pred) < 80%.³ According to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criteria,¹⁶ the severity of COPD was defined as follows: mild, FEV₁% pred ≥ 80%; moderate, 50% ≤ FEV₁% pred < 80%; severe and very severe, FEV₁% pred < 50%.

The distribution of D_LCO per predicted (D_LCO % pred) and the prevalence of low D_LCO by lung function category were evaluated as a cross-sectional analysis using the total cohort. With regard to PRISm, we further investigated the association of low D_LCO with all-cause mortality using 111 patients (the PRISm cohort), excluding 8 patients with no follow-up data. For each case in the PRISm cohort, mortality data were collected from June 1, 2017, to December 31, 2021.

Assessment of the Diffusing Capacity and Transfer Coefficient of the Lungs

D_LCO and D_LCO per alveolar volume (D_LCO/V_A) were measured via the single-breath method using a CHESTAC-8900 spirometer (Chest MI, Inc., Tokyo, Japan) in accordance with the American Thoracic Society/European Respiratory Society (ATS/ERS) guidelines.¹⁷ D_LCO % pred and D_LCO/V_A % pred were calculated using the predicted values of D_LCO and D_LCO/V_A for a person of the same age, gender, and body surface area.¹⁸ In accordance with the clinical review article,¹³ low D_LCO was defined as D_LCO % pred < 80%. In the same manner, low D_LCO/V_A was defined as D_LCO/V_A % pred < 80%. When dividing the PRISm cohort into three groups based on the tertile distribution of D_LCO % pred, the cut-off values were as follows: lowest, < 64.0%; middle, 64.0–85.4%; and highest, ≥ 85.5% for D_LCO % pred.

Clinical Evaluations

For each case, respiratory physicians reviewed the patient’s medical records and assessed the demographic and clinical characteristics: age, gender, height, weight, smoking exposure, and spirometry. Body mass index (BMI; kg/m²) was calculated as weight divided by height squared. Taking into consideration the guidelines for diagnosing obesity in Japanese subjects,¹⁹ obesity, normal weight, and underweight were defined as BMI ≥ 25.0 kg/m², 18.5 to < 25.0 kg/m², and > 18.5 kg/m², respectively. Spirometry was performed before and 15 minutes after BD administration (ie, 200 µg of salbutamol), in line with the guidelines of the Japanese Respiratory Society,²⁰ using CHESTAC-8900. Bronchial reversibility was defined as ≥ 12% and ≥ 200 mL reversibility in post-BD FEV₁. The predicted values of FEV₁ and slow vital capacity (SVC) for a person of the same age, gender, and height were estimated with the equation for the Japanese population.²¹

Statistical Analysis

R software version 4.1.2 (R Foundation for Statistical Computing, Vienna, Austria) was used to perform all statistical analyses. Two-sided P < 0.05 was considered to indicate statistical significance. For baseline characteristics, the heterogeneity in each variable among the lung function categories was evaluated using the analysis of variance (ANOVA), chi-square test, or Kruskal–Wallis test. Tukey’s test, logistic regression analysis, or the Mann–Whitney U-test with Bonferroni correction was used to assess the statistical difference between the PRISm group and any of the other groups. The heterogeneity in the distribution of D_LCO % pred and D_LCO/V_A % pred across the lung-function categories was also analyzed using an ANOVA. Stratified analysis was performed according to BMI levels or smoking status. The prevalences of low D_LCO and low D_LCO/V_A were calculated for each lung-function group and compared using unadjusted and multivariable-adjusted logistic regression models and estimated as odds ratios (ORs) with 95% confidence intervals (95% CIs). Adjustments were made for age, gender, BMI, and smoking exposure. We performed a sensitivity analysis after excluding subjects with bronchial reversibility in order to exclude asthmatic patients. Kaplan–Meier curves were constructed to show the overall survival by the levels of D_LCO % pred and D_LCO/V_A % pred. Log rank testing was performed to study the influence of low D_LCO and low D_LCO/V_A on all-cause mortality. The trend in overall survival according to the tertile groups of D_LCO % pred was assessed with a Cox proportional hazards model. The multivariable-adjusted hazard ratios (HRs) with their 95% CIs of each level of D_LCO % pred or D_LCO/V_A % pred for all-cause death were estimated using a Cox proportional hazards model adjusted for all aforementioned potential confounders. The same model was used to assess the linear trends in the risk of all-cause death across the tertile groups of D_LCO % pred.

Ethical Considerations

The study was approved by the National Hospital Organization Fukuoka National Hospital Institutional Review Board for Clinical Research (#F4-2). The study was conducted in accordance with the Declaration of Helsinki. Informed consent was waived due to the retrospective nature of the study. All the data was anonymized for covering the patient data confidentiality and participant privacy.

Results

Level of D_LCO % Pred and Prevalence of Low D_LCO for Each Lung-Function Group

The prevalence of PRISm was 13.2% among the entire cohort. Table 1 lists the demographic and clinical characteristics of the total cohort. Among the five lung function groups, the prevalence of male gender, the mean age, the mean BMI, and the amount of smoking exposure were not prominent in the PRISm group. Meanwhile, the mean values of absolute SVC and SVC % pred were lowest in the PRISm group.

Table 1 Demographic and Clinical Characteristics of Study Subjects by Lung Function Category

Figure 1 shows the level of D_LCO % pred for each lung-function group. The mean values of D_LCO % pred were 98.4%, 73.9%, 99.3%, 89.4%, and 71.0% in the preserved-spirometry group, the PRISm group, the mild-COPD group, the moderate-COPD group, and the severe/very severe-COPD group, respectively. In the PRISm group, D_LCO % pred was significantly lower than in the other lung function groups except for in the severe/very severe-COPD group. Likewise, the prevalence of low D_LCO was significantly higher in subjects with PRISm than in those with preserved spirometry, mild COPD, or moderate COPD (all P < 0.01); it was as high as about 60% in the PRISm group and the severe/very severe-COPD group (Figure 2). The results were substantially similar after adjustments for potential confounders; there was a significant increase in OR in the PRISm group as compared to the preserved-spirometry group, the mild-COPD group, and the moderate-COPD group (all P < 0.01) (Table 2). Broadly similar results were obtained in the analysis stratified by BMI levels (Supplementary Figures S1 and S2) or smoking status (Supplementary Figures S3 and S4). The results were not substantially changed after excluding asthmatic subjects (Supplementary Figures S5 and S6). Further, there was no significant decrease in the level of D_LCO/V_A % pred or increase in the prevalence of low D_LCO/V_A in the PRISm group compared to the group with preserved spirometry, mild COPD, or moderate COPD (all P > 0.05) (Supplementary Figures S7 and S8).

Table 2 Multivariable-Adjusted Regression Analysis for Low DLCO in the Patient Groups Based on FEV1 Value

Figure 1 Level of DLCO % pred by lung-function group. Abbreviations: DLCO % pred, diffusing capacity of the lung…